A light-skinned “mulatto” woman and personal servant, known for her beauty and nurturing nature, who is determined to protect her young son from being sold by running away to freedom.

The intelligent and resourceful husband of Eliza Harris, who is determined to escape to Canada and build a life free from slavery for himself and his family.

The kind and loving young daughter of Augustine St. Clare, whose innocence and pious nature make her a beacon of hope and love for others around her.

A brutal and cruel plantation owner known for his desire to "break" his slaves and his proclivity for physical violence against them.

The wife of Arthur Shelby and a compassionate mistress who raises her servants according to Christian values and disapproves of the cruelty of slavery.

A young slave girl who is mischievous and considered incorrigible, yet changes through the love and kindness shown to her by others.

The financially troubled owner of Uncle Tom, who is forced to sell his slaves despite his personal reservations and is seen as a symbol of the flawed and hypocritical "kind" master.

Uncle Tom’s second master, who is a wealthy and indulgent man conflicted about the morality of slavery but too complacent to take significant action against it.

Augustine’s strict, efficient Northern cousin who opposes slavery ideologically and struggles with her own prejudices while learning to love and educate Topsy.

A slave trader who believes himself to be humane, yet is opportunistic and primarily driven by profit in the trading of enslaved people.

A rugged headhunter initially hired to capture runaway slaves who undergoes a personal transformation after an encounter with the fugitives he pursues.