A young, independent schoolteacher from Vermont who moves to Wyoming Territory, where she navigates her emotions and societal expectations while forming a significant relationship with the Virginian.

An Eastern visitor to Wyoming who observes and participates in the Virginian's adventures, offering insights into frontier life through his detailed perspective.

An antagonist who initially tries to intimidate the Virginian and continues to be a thorn in his side, representing the threats posed by unscrupulous individuals in the wilderness.

A rancher known for his harsh treatment of horses, demonstrating the dangerous potential of becoming wild and cruel without the restraint of civilized behavior.

A dim-witted but kind-hearted cowboy who falls prey to unfortunate circumstances, embodying the struggles faced by those with limited options on the frontier.

Once a friend of the Virginian, he becomes involved in cattle rustling, presenting a moral challenge for the Virginian as their paths diverge.

Owners of the Sunk Creek Ranch, who support and counsel their employees, providing guidance to characters such as the Virginian and Molly.

Molly's neighbor and confidante in Bear Creek, who offers advice and support, particularly during Molly's relationship challenges.

Molly’s supportive family member from New Hampshire, who encourages her independence and personal choices.

A cook at the Sunk Creek Ranch who befriends the Virginian, offering perceptive commentary on the unfolding events and characters.

A wealthy bachelor from Vermont who is interested in Molly Wood, providing contrast to the Virginian due to his suitability in the eyes of society.