The 12-year-old heir to the Kingdom of Dor, characterized by her empathy and kindness, who forms a bond with Despereaux. She plays a pivotal role in the kingdom's dynamics.

A rat fascinated by light yet condemned to darkness, whose conflicting nature sets in motion significant events in the story. His full name reflects a balance of darkness and light.

A girl with dreams of royalty who faces numerous hardships throughout her life. Her involvement with the main plot underscores her longing for love and recognition.

An archetypical rat notorious for his obsession with misery and suffering. Known for his cunning nature and serves as Roscuro's only friend, guiding Despereaux during a crucial scene.

The longtime jailer of the castle dungeon, capable of navigating its dark maze. His interaction with Despereaux is influenced by his memories of the kingdom's past.

Responsible for the "red thread of death" used on condemned mice. He assists Despereaux with tools for his quest and shares an appreciation for fairy tales.

Despereaux's dramatic French mother who expresses disappointment with her offspring. Her actions during Despereaux's trial are more theatrical than sincere.

Despereaux’s father, initially ashamed of his son and participates in his trial. He later regrets his lack of support and seeks forgiveness.