A mysterious and seductive figure who initially infiltrates the monastery disguised as a young monk named "Rosario" and reveals herself to Ambrosio.

A young and beautiful girl, noted for her innocence and purity, who is the object of Don Lorenzo's affection.

A noblewoman and sister of Don Lorenzo, who finds herself entangled in a forced religious life contrary to her desires.

A noble Spanish gentleman and brother to Agnes, representing the virtues of traditional and youthful Romantic masculinity.

A nobleman and Agnes's lover who faces adversity and danger during his travels, temporarily living under the guise of a commoner.

The devoted valet of Don Raymond, involved in various schemes throughout the narrative, and an accomplished poet and musician.

The intelligent and protective mother of Antonia, who holds conservative views and takes extreme measures to preserve her daughter's purity.

The authoritative head of the Convent of St. Clare, who symbolizes religious corruption and is known for her merciless disposition.