Matthew is a central figure in the play, depicted as a kind and open-minded university student with aspirations in human rights. His tragic story serves as a catalyst for broader discussions on hate crimes and societal change.

The bartender at The Fireside bar, Matt is deeply affected by the events and feels regret about not having recognized the danger Matthew was in. He opposes the death penalty, advocating for justice without revenge.

A policewoman who responded to the emergency call, Reggie is faced with the personal consequence of possible HIV exposure during her attempt to save Matthew. She remains hopeful for a future where Laramie moves beyond the tragedy.

As the head of the Theatre Department at the University of Wyoming, Rebecca sees it as her duty to educate her students about homophobia, partially through staging plays that challenge societal views.

A theatre student with a complicated view on homosexuality, Jedadiah experiences personal growth and reevaluates his beliefs throughout the play, leading to increased empathy and understanding.

The lead investigator on Matthew Shepard’s case, Rob grapples with the emotional weight of the investigation and is eventually moved to confront and combat discrimination as a result of it.

A close friend of Matthew’s, Romaine is an active voice against the idea of the crime being purely motivated by theft, and she organizes actions to counterhate speech in the aftermath of the tragedy.

A local limousine driver familiar with Matthew, Doc provides contextual information about Laramie and unknowingly sheds light on the subtle nature of biases in the town.

Reggie Fluty's mother, Marge provides local insight and opposes blaming Matthew for the repercussions faced by her daughter, instead focusing on those who committed the crime.

A religious figure who, despite recognizing the wrongdoing in Matthew's murder, lacks empathy and is critical of Matthew's lifestyle.

A religious leader who extends compassion and understanding towards Matthew and emphasizes the broader implications of homophobic attitudes within the community.

Matthew's attackers, characterized by information from others in the community, are depicted as frustrated, insecure young men influenced by their societal environment.