A servant to Vermandero, born a gentleman, who harbors an obsessive desire for Beatrice, complicating the dynamics of the household.

A nobleman from Valencia who falls in love with Beatrice at first sight and becomes romantically entangled with her.

A nobleman who owns the castle where the play is set and is the father of Beatrice, controlling her marital prospects.

Beatrice's waiting-woman who becomes involved in Beatrice's romantic schemes.

A friend of Alsemero who aids in arranging meetings for Beatrice and Alsemero, with his romantic interest in Diaphanta.

Betrothed to Beatrice at the start of the play and unsuspectingly drawn into the darker schemes of other characters.

Brother of Alonzo, who seeks vengeance following the events that impact his family.

The young wife of Alibius, who is pursued by multiple admirers yet remains faithful.

Disguises himself in an asylum to pursue Isabella, exemplifying the theme of uncertain identities in the play.