Charlotte is a wise and clever spider who becomes Wilbur's best friend, using her web-spinning abilities to influence events on the farm in subtle yet profound ways.

Templeton is a large, selfish rat living on the farm, often indulging in his own interests but occasionally assisting Wilbur and Charlotte when incentivized.

Fern is an eight-year-old girl who first saves Wilbur's life, forming a special bond with him and experiencing a summer filled with childhood innocence and growth.

Mr. Zuckerman is the owner of the farm where Wilbur lives, drawn into the excitement surrounding Wilbur's perceived uniqueness after seeing messages in Charlotte's web.

Goose is a farm inhabitant who tends her eggs and creates an undercurrent of chatter and commentary, often repeating herself and adding to the barnyard dynamics.

Doctor Dorian is the county doctor who provides reassurances to Mrs. Arable about Fern, offering a calm and open-minded perspective on the events at the farm.

The old sheep on the farm delivers news and reality checks in a harsh manner, catalyzing important developments in the story.

Lurvy is the dedicated farmhand who caters to Mr. Zuckerman's needs and marvels at the mysterious messages appearing in Charlotte's web.

Mrs. Arable is Fern's concerned mother, often worried about Fern's tales of talking animals and her time at the farm.

Mr. Arable is Fern's understanding and lenient father who initially plans to slaughter Wilbur but then allows Fern to care for him.

Avery is Fern's energetic brother, often getting into mischief and seeking his own adventures alongside Fern.