An unnamed character representing Dion Boucicault, the historical playwright, who appears as both a helper and a satirical figure, playing an Indigenous stereotype in the play to critique the historical representation and influence of melodrama.

An Indian character who plays the roles of enslaved individuals, contributing to the play's exploration of racial identity and stereotypes while offering commentary on racial representation in theatrical roles.

The "octoroon" character central to the play's title, whose mixed racial heritage and unique position in society reflect the complicated themes of race and identity explored in the narrative.

A wealthy heiress and neighbor to the Peytons, who is infatuated with George and embodies the exaggerated performance of white femininity and societal norms of the time.

Two enslaved women portrayed by Black actresses, whose candid conversations and aspirations highlight the harsher truths of plantation life and provide insight into the reality of their existence.